anti human tuba1b antibody  (Proteintech)

Journal: bioRxiv

Article Title: The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental development

doi: 10.1101/2024.03.01.582942

Figure Lengend Snippet: a Overview of placental age stages used for the study. Karyotypes are indicated by the key. wpc, weeks post conception. b Expression of the X inactivation regulator XIST , bulk RNA-sequencing (RNA-seq) counts in the 45,X placenta group (n=6) compared to 46,XX placenta (n=6) and 46,XY placenta (n=6). c Principal component analysis (PCA) of 45,X, 46,XX and 46,XY placental samples used in the study. PC, principal component. d Volcano plot showing differential expression of genes between the 45,X and 46,XX matched control samples (n=4 in each group). The top ten most differentially expressed genes in each dataset are labeled, based on adjusted p-value (p-adj) and where log 2 fold change (FC) is greater than +/-0.7. Genes with higher expression in 45,X samples have a positive log 2 FC and those with higher expression in control tissues have a negative log 2 FC. The significance level of highlighted points is shown in the key. e Volcano plot showing differential expression of genes between the 45,X and 46,XY matched control samples. f Venn diagram with the common intersection showing placenta-specific genes that are consistently lower in 45,X. Data were generated for each group (n=6 versus n=6) with a differential expression cut-off of log 2 FC>0.5 and p-adj <0.05. g Violin plot of CSF2RA expression (normalized counts) in the placenta (bulk RNA-seq) (n=6 each group). h Violin plot of AADACL3 expression (normalized counts) in the placenta (bulk RNA-seq) (n=6 each group). i Heat map of AADACL3 and CSF2RA expression across all placental samples (n=6 samples in each group).

Article Snippet: In brief, antigen retrieval was performed to unmask the epitope (Heat Induced Epitope Retrieval (HIER), Bond-max protocol F), endogenous activity was blocked with peroxidase using a Bond polymer refine kit (cat # DS9800), then incubation was undertaken with a primary rabbit polyclonal CSF2RA (GM-CSF receptor alpha) antibody for 1 hour (Origene TA323990S, 1:100 dilution, HIER1 for 30 mins).

Techniques: Expressing, RNA Sequencing Assay, Control, Labeling, Generated

Journal: bioRxiv

Article Title: The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental development

doi: 10.1101/2024.03.01.582942

Figure Lengend Snippet: a Consensus first trimester single cell data Uniform Manifold Approximation and Projection (UMAP) for clusters based on cell type ( left panel ) and feature plot for CSF2RA ( right panel ). These data were generated by the Vento-Tormo/Teichmann groups at the Wellcome Sanger Institute, Hinxton, UK and can be accessed using CZ CELLxGENE from https://maternal-fetal-interface.cellgeni.sanger.ac.uk/ (Vento-Tormo, R., Efremova, M., Botting, R.A. et al. Single-cell reconstruction of the early maternal–fetal interface in humans. Nature 563, 347–353 (2018); https://doi.org/10.1038/s41586-018-0698-6 ). This graphic is generated under a Creative Commons Attribution-BY 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ ). DC, dendritic cells; dM, decidual macrophages; dS, decidual stromal cells; Endo, endothelial cells; Epi, epithelial glandular cells; EVT, extravillous trophoblast; f, fetal; F, fibroblasts; HB, Hofbauer cells; ILC, innate lymphocyte cells; l, lymphatic; m, maternal; M3, maternal macrophages; PV, perivascular cells; p, proliferative; SCT, syncytiotrophoblast; VCT, villous cytotrophoblast. b H&E staining of 45,X placenta at 11 weeks post conception (wpc) ( upper panel ), 46,XY placenta at 14 wpc ( center panel ) and 45,X placenta at 14 wpc) ( lower panel ) (scale bars 50 μm). Arrowheads show regions of irregular villus border in the 14wpc 45,X sample. c Immunohistochemistry of CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) in 46,XX, 46,XY and 45,X placenta at 14wpc at lower magnification ( left panels ) (scale bars 100 μm) and at higher magnification ( right panels ) (scale bars 50 μm). Arrowheads show regions of irregular border and diffuse CSF2RA staining in the 14wpc 45,X villus. Inset images show higher magnification of regions indicated by an asterix. d Biological process enrichment analysis for genes lower in 45,X placenta compared to 46,XY controls (bulk RNA-sequencing n=6 each group; 266 genes identified) with a differential expression cut-off of log 2 FC>0.5 and adjusted p-value <0.05.

Article Snippet: In brief, antigen retrieval was performed to unmask the epitope (Heat Induced Epitope Retrieval (HIER), Bond-max protocol F), endogenous activity was blocked with peroxidase using a Bond polymer refine kit (cat # DS9800), then incubation was undertaken with a primary rabbit polyclonal CSF2RA (GM-CSF receptor alpha) antibody for 1 hour (Origene TA323990S, 1:100 dilution, HIER1 for 30 mins).

Techniques: Generated, Staining, Immunohistochemistry, RNA Sequencing Assay, Expressing

Journal: BioMed Research International

Article Title: Type I Collagen Suspension Induces Neocollagenesis and Myodifferentiation in Fibroblasts In Vitro

doi: 10.1155/2020/6093974

Figure Lengend Snippet: Effect of EC-I treatment on collagen type I and III and HSP47/P4HA1 expression. (a) Representative immunofluorescence images of NIH 3T3 cells treated for 24 h with EC-I suspension (4 mg/ml) and stained with antibodies against collagen type I or type III (green). Nuclei were counterstained with DAPI (blue) (magnification 100x). The results are representative of three independent experiments in duplicate. (b) Immunoblotting assay for HSP47 and P4HA1. Following densitometric analysis, obtained values were normalized vs . β -actin. Data are from three independent experiments in duplicate, and values are expressed as mean ± SEM. ∗∗ P < 0.01 vs . control. A representative immunoblot of HSP47 and P4HA1 is also shown.

Article Snippet: Membranes were blocked with 5% nonfat dry milk for 1 h at room temperature and then incubated overnight at 4°C with rabbit monoclonal antibody anti-heat shock protein 47 (HSP47) 1 : 1,000, goat polyclonal antibody anti-prolyl 4-hydroxylase subunit alpha-1 (P4HA1) 1 : 1,000, rabbit monoclonal antibody anti- α -actin smooth muscle (ACTA2, α -SMA) 1 : 1,000 (OriGene, Rockville, Maryland, USA), or with goat polyclonal antibody anti- β -actin 1 : 1,000 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA.).

Techniques: Expressing, Immunofluorescence, Staining, Western Blot

Journal: Respiratory Research

Article Title: Glutathione-S-transferases in lung and sputum specimens, effects of smoking and COPD severity

doi: 10.1186/1465-9921-9-80

Figure Lengend Snippet: Immunohistochemical staining for GST alpha in specimens from the central and peripheral lung of a non-smoker (A and B, respectively), a smoker (C and D), and patients with Stage I-II COPD (E and F) and Stage IV COPD (G and H) . GST alpha was mainly located in the airway epithelium.

Article Snippet: The sections were incubated with the primary polyclonal antirabbit antibodies for GST mu (1:100), GST pi (1:100) or GST alpha 1:75 (Acris Antibodies, Hiddenhausen, Germany).

Techniques: Immunohistochemical staining, Staining

Journal: Respiratory Research

Article Title: Glutathione-S-transferases in lung and sputum specimens, effects of smoking and COPD severity

doi: 10.1186/1465-9921-9-80

Figure Lengend Snippet: GST alpha expression in the airways and in total lung homogenates . A. The GST alpha immunoreactivity in airway epithelium of central and peripheral airways. The staining was graded as negative (0), weak (1) or moderate (2) or intense (3). The GST alpha immunoreactivity was strong in the epithelium of both large, cartilaginous airways as well as in the epithelium of small peripheral bronchioli. There was a trend for diminished immunoreactivity in cases of very severe (Stage IV) COPD but the difference between the groups was not statistically significant. The means are shown as columns with error bars representing SEM. B. The percentage of GST alpha positive epithelial cells was observed to decrease in the large airways of the patients with very severe (Stage IV) COPD compared to non-smokers (p = 0.02). C. Western analysis of GST alpha in the lung homogenates of healthy non-smokers and smokers and in patients with different stages of COPD showed an increased immunoreactivity in patients with Stage I-II COPD compared to non-smokers or smokers (p = 0.007). The means of the measured sum intensities are shown as columns with error bars representing SEM.

Article Snippet: The sections were incubated with the primary polyclonal antirabbit antibodies for GST mu (1:100), GST pi (1:100) or GST alpha 1:75 (Acris Antibodies, Hiddenhausen, Germany).

Techniques: Expressing, Staining, Western Blot

Journal: Respiratory Research

Article Title: Glutathione-S-transferases in lung and sputum specimens, effects of smoking and COPD severity

doi: 10.1186/1465-9921-9-80

Figure Lengend Snippet: GST alpha immunoreactivity in sputum cells and supernatants . A. Western analysis for GST alpha in induced sputum supernatants revealed an increased immunoreactivity in patients with chronic bronchitis and in Stage II-III COPD compared to healthy non-smokers (p < 0.001). The means of the measured intensities are shown as columns with error bars representing SEM. B. Representative sputum cytospins from a smoker (a), patient with chronic bronchitis (b) and patient with Stage II COPD (c). Macrophages in the induced sputum exhibited positive GST alpha reactivity.

Article Snippet: The sections were incubated with the primary polyclonal antirabbit antibodies for GST mu (1:100), GST pi (1:100) or GST alpha 1:75 (Acris Antibodies, Hiddenhausen, Germany).

Techniques: Western Blot

Journal: Case Reports in Pathology

Article Title: Granulomatous Pancreatitis in a Patient with Acute Manifested Insulin-Dependent Diabetes Mellitus

doi: 10.1155/2014/615426

Figure Lengend Snippet: Antibodies and their dilutions used in the study.

Article Snippet: Anti-alpha-1 , Mouse monoclonal , Acris Antibodies , 1 : 400.

Techniques: Diagnostic Assay

Journal: Biomedicines

Article Title: Unravelling the Role of PAX2 Mutation in Human Focal Segmental Glomerulosclerosis

doi: 10.3390/biomedicines9121808

Figure Lengend Snippet: List of primary antibodies.

Article Snippet: Alpha-Actinin 4 , Origene , TA307264 , 1:200.

Techniques: